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DNA topoisomerase 1 is a topoisomerase enzyme that catalyzes ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. It catalyzes reaction that leads to the conversion of one topological isomer of DNA to another.

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Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor.

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